abstract：:Brain proteins extractable with distilled water or 9 mol/L urea were subjected to two-dimensional gel electrophoresis. They are examined in relation to the identification of actin, tubulin, neurofilament proteins, glial fibrillary acidic protein; proteins of human embryonic neocortex, synaptosomes, myelin, and plasma;...

journal_title：Clinical chemistry

authors： Comings DE,Carraway NG,Pekkula-Flagan A

更新日期：1982-04-01 00:00:00

abstract：:We describe a direct radioimmunoassay for serotonin (5-hydroxytryptamine) in cerebrospinal fluid, platelet-poor plasma, and serum. We raised antisera in rabbits against serotonin diazotized to a conjugate of bovine albumin and D,L-p-aminophenylalanine. Polyethylene glycol, alone or in combination with anti-rabbit immu...

journal_title：Clinical chemistry

authors： Engbaek F,Voldby B

更新日期：1982-04-01 00:00:00

abstract：:Radioimmunoassay of triiodothyronine was performed with a homogeneous incubation system. Bound and free ligand were separated by partitioning in an aqueous two-phase system. The agreement between the results obtained with this technique and conventional heterogeneous radioimmunoassay was very good (r = 0.981). ...

journal_title：Clinical chemistry

authors： Mattiasson B,Eriksson H

更新日期：1982-04-01 00:00:00

abstract：:A total of 1357 polypeptides [946 acidic (isoelectric focusing) and 411 basic (nonequilibrium pH-gradient electrophoresis)] from human HeLa cells have been separated and catalogued with use of high-resolution two-dimensional gel electrophoresis. Of these polypeptides, 1266 were detected by labeling cells with [35S]met...

journal_title：Clinical chemistry

authors： Bravo R,Celis JE

更新日期：1982-04-01 00:00:00

abstract：:Two-dimensional protein maps of biopsies from normal mucosa, tubular adenomas (polyps), and colonic carcinomas in humans have been studied in order to obtain information on the possible precancerous role of intestinal polyps. The maps are complex and several hundred protein spots are seen after staining with Coomassie...

journal_title：Clinical chemistry

authors： Thorsrud AK,Vatn MH,Jellum E

更新日期：1982-04-01 00:00:00

abstract：:We tested a new amylase reagent involving chromogenic substrates (Pantrak, Calbiochem-Behring) for precision, dynamic range, and susceptibility to potential interferences. Two p-nitrophenyl-alpha-maltaosides are used as substrates, which amylase cleaves to shorter-chain p-nitrophenyl maltaosides, the latter then yield...

journal_title：Clinical chemistry

authors： Fenton J,Foery R,Piatt L,Geschwindt K

更新日期：1982-04-01 00:00:00

abstract：:We examined the comparative behavior of subject-specific multivariate and univariate reference regions, using both computer-generated data and serial (semi-annual) measurements of selected analytes in subjects from a large health-maintenance program. Univariate studies under both homeostatic and random-walk time-serie...

journal_title：Clinical chemistry

authors： Harris EK,Yasaka T,Horton MR,Shakarji G

更新日期：1982-03-01 00:00:00

abstract：:Zinc is determined in neutrophils and lymphocytes (isolated from whole blood on discontinuous gradients of Ficoll-Hypaque) and in microliter quantities of plasma and erythrocytes by flameless atomic absorption spectrophotometry with greater sensitivity than with conventional flame atomic absorption spectrophotometry. ...

journal_title：Clinical chemistry

authors： Whitehouse RC,Prasad AS,Rabbani PI,Cossack ZT

更新日期：1982-03-01 00:00:00

abstract：:We compared three radioligands for use in a cytoplasmic estrogen-receptor assay, using pooled cytosol from human breast adenocarcinomas. The estrogen receptor content was determined in vitro by a dextran-coated charcoal method involving a 4-h incubation with and without diethylstilbestrol. Tritiated moxestrol failed t...

journal_title：Clinical chemistry

authors： Pieslor PC,Gibson RE,Eckelman WC,Oates KK,Cook B,Reba RC

更新日期：1982-03-01 00:00:00

abstract：:We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tra...

journal_title：Clinical chemistry

authors： Duncan P,Gochman N,Bayse D,Burnett R,Carey N,Carter R,Fellows WD,Garber C,Kessler G,McComb R,Miller W,Nast P,Ryan W,Schaffer R,Tejeda B,Vanderlinde R,Widdowson G

更新日期：1982-02-01 00:00:00

abstract：:We describe an improved "high-pressure" liquid-chromatographic assay for simultaneous determination in serum of the five major antiepileptic drugs (ethosuximide, primidone, phenobarbital, phenytoin, and carbamazepine) and N-desmethylmethsuximide (the compound that must be quantitated for therapeutic drug monitoring of...

journal_title：Clinical chemistry

authors： Szabo GK,Browne TR

更新日期：1982-01-01 00:00:00

abstract：:Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nea...

journal_title：Clinical chemistry

authors： Acton WJ,Van Duyn OM,Allen LV,Flournoy DJ

更新日期：1982-01-01 00:00:00

abstract：:We evaluated a new multiple-channel chemistry analyzer, the Boehringer Mannheim "Diagnostic M." This instrument can perform 25 tests at the rate of 120 1.3-mL serum samples per hour. The instrument may be run in either a profile mode or single-test mode. In the single-test mode only the necessary reagent is pumped. th...

journal_title：Clinical chemistry

authors： Robinson CA,Proelss H,Stabler TV

更新日期：1982-01-01 00:00:00

abstract：:Two patients with Paget's disease of bone were subjected to plasmapheresis. Alkaline phosphatase activities of serum declined sharply, but returned to preplasmapheresis values within eight to 10 days. The biological half-life of circulating skeletal alkaline phosphatase, as calculated from these experiments, is betwee...

journal_title：Clinical chemistry

authors： Posen S,Grunstein HS

更新日期：1982-01-01 00:00:00

abstract：:Determination of the MB isoenzyme of creatine kinase in patients with acute myocardial infarction may be disturbed by the presence of macro creatine kinase. The relative molecular mass of this form of creatine kinase in human serum is at least threefold that of the ordinary enzyme, and it is more thermostable. Here we...

journal_title：Clinical chemistry

authors： Stein W,Bohner J,Steinhart R,Eggstein M

更新日期：1982-01-01 00:00:00

abstract：:We describe a sensitive method for the determination of vanadium in biological substances by atomic absorption spectroscopy. By using background correction and pyrolytically coated graphite tubes with flameless apparatus, this vanadium assay has a sensitivity of 65 pg and a detection limit of 30 pg, approximately sixf...

journal_title：Clinical chemistry

authors： Stroop SD,Helinek G,Greene HL

更新日期：1982-01-01 00:00:00

abstract：:We examined the relationship in a large-living population between the concentration of triglycerides in plasma from fasting persons and the concentration of very-low-density lipoprotein cholesterol (VLDL-C) as determined after preparative ultracentrifugation. Data from individuals in the Framingham Heart Study who wer...

journal_title：Clinical chemistry

authors： Wilson PW,Abbott RD,Garrison RJ,Castelli WP

更新日期：1981-12-01 00:00:00

abstract：:We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactiv...

journal_title：Clinical chemistry

authors： Lee TP,Tan CH

更新日期：1981-12-01 00:00:00

abstract：:We describe a fully automated method of analysis for plasma epinephrine and norepinephrine by use of a two-column system of "high-performance" liquid chromatography. Catecholamines in deproteinized plasma are purified on the first (preparation) column, then transferred automatically to the second (analytical) column i...

journal_title：Clinical chemistry

authors： Yamatodani A,Wada H

更新日期：1981-12-01 00:00:00

abstract：:Antiserum performance in a nephelometric system can be characterized by parameters derived from measuring reaction rates. The characterization process is derived from a series of dose-response curves (elicited nephelometric response vs antigen concentration) generated from various dilutions of the antiserum being test...

journal_title：Clinical chemistry

authors： Hudson GA,Ritchie RF,Haddow JE

更新日期：1981-11-01 00:00:00

abstract：:We describe a method for determination of guanidino compounds in plasma and erythrocytes. The compounds are separated by liquid chromatography, the concentrations in the effluent being estimated fluorometrically (Trans. Am. Soc. Artif. Intern. Organs 24: 61, 1978). Guanidinoacetic acid, guanidinosuccinic acid, guanidi...

journal_title：Clinical chemistry

authors： Kikuchi T,Orita Y,Ando A,Mikami H,Fujii M,Okada A,Abe H

更新日期：1981-11-01 00:00:00

abstract：:This simple, accurate, and reproducible colorimetric method for determining the activity of angiotensin-I converting enzyme is based upon colorimetry of the quinoneimine dye produced from the substrate p-hydroxyhippuryl-L-histidyl-L-leucine by action of this enzyme through the following series of reactions. The enzyme...

journal_title：Clinical chemistry

authors： Kasahara Y,Ashihara Y

更新日期：1981-11-01 00:00:00

abstract：:A new enzymic method is described for the determination of creatine in urine by continuous-flow analysis. The measurement is accomplished by transforming creatine to formaldehyde in reactions catalyzed by creatinase (creatine amidinohydrolase) and sarcosine dehydrogenase. The formaldehyde is reacted with 4-amino-3-hyd...

journal_title：Clinical chemistry

authors： Yasuhara M,Fujita S,Furukawa I,Arisue K,Kohda K,Hayashi C

更新日期：1981-11-01 00:00:00

abstract：:We evaluated and compared five commercial radioimmunoassay kits with a standard counter-immunoelectrophoretic assay for the measurement of prostatic acid phosphatase in serum. Four of the five radioimmunoassays performed as described by the supplier with respect to sensitivity, stability, precision, linearity, analyti...

journal_title：Clinical chemistry

authors： Wright GL Jr,Schellhammer PF,Brassil DN,Sieg SM,Leffell MS

更新日期：1981-10-01 00:00:00

abstract：:The concentration of the conjugated bile acid, cholylglycine, in serum is a sensitive and specific indicator of hepatic function. We describe a convenient, specific, and precise radioimmunoassay for cholylglycine, in which 125I-labeled cholylglycyltyrosine is used as tracer. In addition, a blocking agent in the buffer...

journal_title：Clinical chemistry

authors： Miller P,Weiss S,Cornell M,Dockery J

更新日期：1981-10-01 00:00:00

abstract：:The metabolism of the synthetic substrate 7-ethoxyresorufin is a selective measure of the activity of cytochrome P-448 monooxygenase, the subset of cytochrome P-450-mediated enzymes preferentially induced by polycyclic aromatic hydrocarbons and related compounds. 7-Ethoxycoumarin metabolism, on the other hand, reflect...

journal_title：Clinical chemistry

authors： Bostick WD,Kao J,Holland JM,Mrochek JE

更新日期：1981-09-01 00:00:00

abstract：:The advent of hybridoma technology has provided the immunotechnologist a finely tunable instrument that should permit a marked advance in the immunologic sciences. The ability to choose the precise antibody required and the virtually unlimited availability of easily purified antibodies have already resulted in the sim...

journal_title：Clinical chemistry

authors： David GS,Wang R,Bartholomew R,Sevier ED,Adams TH,Greene HE

更新日期：1981-09-01 00:00:00

abstract：:We report the production and comparative specificities of antisera raised to different immunogens containing codeine, morphine, and oxycodone. Antisera raised to bovine serum albumin (BSA) conjugates of codeine-6-hemisuccinate, ethylmorphine-6-hemisuccinate, or oxycodone-6-carboxymethyloxime had greatest recognition o...

journal_title：Clinical chemistry

authors： Findlay JW,Butz RF,Jones EC

更新日期：1981-09-01 00:00:00

abstract：:We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight wit...

journal_title：Clinical chemistry

authors： Miyai K,Ishibashi K,Kawashima M

更新日期：1981-08-01 00:00:00

abstract：:Total bilirubin is determined here with a multipoint calibration approach and with use of Standard Reference Material No. 916 bilirubin from the National Bureau of Standards. The bilirubin calibration solutions prepared in solutions of Cohn Fraction V human albumin were stable for at least one year when stored at -80 ...

journal_title：Clinical chemistry

authors： Garber CC

更新日期：1981-08-01 00:00:00

abstract：:We determined the LD-1/LD-2 isoenzyme ratio in hemolysates of erythrocytes by electrophoresis on cellulose acetate and on agarose. A ratio exceeding 1.0 was found with the former but not the latter. Results were similar for in vitro models of hemolytic disorders. Using cellulose acetate electrophoresis, we determined ...

journal_title：Clinical chemistry

authors： Van Lente F,Marchand A,Galen RS

更新日期：1981-08-01 00:00:00

abstract：:Radioimmunoassays for haloperidol are described, involving use of tritium- or 125I-labeled drug or tritium-labeled spiroperidol, and a rabbit antiserum to a drug/bovine serum albumin conjugate. The 125I-labeled drug was prepared by the Chloramine T iodination technique. A fluoroimmunoassay for haloperidol is also desc...

journal_title：Clinical chemistry

authors： Rowell FJ,Hui SM,Kamel SR

更新日期：1981-07-01 00:00:00

abstract：:An optimized assay for alkaline phosphatase (EC 3.1.3.1) is reported. A discrete analyzer, the DuPont Automatic Clinical Analyzer (aca), was used for this study. The assay is based on results of response-surface experimental co-optimization techniques, and response is enhanced over the present aca assay. A key feature...

journal_title：Clinical chemistry

authors： Thompson JC,Hodges CT,Dobler GL,Williamson JA Jr

更新日期：1981-07-01 00:00:00

abstract：:17 alpha-Hydroxyprogesterone, cortisol, and 11-deoxycortisol were extracted with methylene chloride, separated by liquid chromatography, identified by their retention times, and quantitated by monitoring the absorbance at 254 nm. The method is specific and sensitive to as little as 2.5 ng of 17 alpha-hydroxyprogestero...

journal_title：Clinical chemistry

authors： Canalis E,Caldarella AM,Reardon GE

更新日期：1981-07-01 00:00:00

abstract：:A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without...

journal_title：Clinical chemistry

authors： Ohkubo A,Kamei S,Yamanaka M,Arai F,Kitajima M,Kondo A

更新日期：1981-07-01 00:00:00

abstract：:We review the following labels used as substitutes for radioisotopes in immunoassay systems: bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performi...

journal_title：Clinical chemistry

authors： Schall RF Jr,Tenoso HJ

更新日期：1981-07-01 00:00:00

abstract：:This highly sensitive method for determination of beta 2-microglobulin (beta 2-m) in human urine or serum is based on direct agglutination by beta 2-m of latex particles on which an antibody against beta 2-m is adsorbed. The agglutination is quantified by counting the remaining unagglutinated particles, or by turbidim...

journal_title：Clinical chemistry

authors： Bernard AM,Vyskocil A,Lauwerys RR

更新日期：1981-06-01 00:00:00

abstract：:A new radiometric assay specific for creatine kinase isoenzyme MB was evaluated with respect to its precision and agreement with a conventional electrophoretic CK-MB assay for the diagnosis of myocardial infarction. The reference interval we find for serum CK-MB in healthy subjects is 0--30 micrograms/L. The coefficie...

journal_title：Clinical chemistry

authors： Kwong TC,Rothbard RL,Biddle TL

更新日期：1981-06-01 00:00:00

abstract：:A method is described for separating antibody-bound from free fractions in ligand assays by column affinity chromatography, and its application to radioimmunoassay of choriomammotropin. In the method, 70 x 10 mm (i.d.) polypropylene columns containing about 150 mg of immunosorbent (goat anti-rabbit gamma-globulins cov...

journal_title：Clinical chemistry

authors： Cornale P,Bonazzi M,Multinu C,Romelli P,Vancheri L,Pennisi F

更新日期：1981-06-01 00:00:00

abstract：:We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensit...

journal_title：Clinical chemistry

authors： Worah D,Yeung KK,Ward FE,Carrico RJ

更新日期：1981-05-01 00:00:00